Decrease in TTP pools mediated by 5-bromo-2'-deoxyuridine exposure in a human glioblastoma cell line.

Down-Regulation of CD14 Transcripts in Human Glioblastoma Cell Line U87 MG

Background: Pattern recognition receptors (PRRs) are the main sensors of pathogen and danger signals in innate immunity of which Toll Like Receptors (TLRs) are the most studied ones. The contribution of PRRs in cerebral inflammation induced by microbial infection, tissue damage and cancer has not extensively been addressed so far. Glioma is the most common tumor of the central nervous system an...

Abundant Non-Canonical dUTP Found in Primary Human Macrophages Drives Its Frequent Incorporation by Human Immunodeficiency Virus Type 1 Reverse Transcriptase

Terminally differentiated/non-dividing macrophages contain extremely low cellular dNTP concentrations (20-40 nM), compared to activated CD4 + T cells (2-5 M). However, our LC-MS/MS study revealed that the non-canonical dUTP concentration (2.9 M) is ~60 times higher than TTP in macrophages, while the concentrations of dUTP and TTP in dividing human primary lymphocytes are very similar. Specifi...

Gene Expression Quantification of Toll like Receptors 2, 4 and Co-molecules in Human Glioblastoma Cell Line (U87-MG): Toward a New In vitro Model of Inflammation

Evaluation of (5R,6R)-5-Bromo-6-Ethoxy-5-Ethyl-5,6-Dihydro-2\'- Deoxyuridine as a Brain-Targeted Prodrug of 5-Ethyl-2\'- Deoxyuridine

(+)-Trans-(5R,6R)-5-bromo-6-ethoxy-5-ethyl-5,6-dihydro-2'-deoxyuridine [(5R,6R)-BEEDU], a po‌tential brain-targeted prodrug of 5-ethyl-2'-deoxyuridine (EDU), was synthesized by the regiospecific addition of BrOEt to the 5,6-olefinic bond of EDU. (5R,6R)-BEEDU is more lipophilic (log P = 0.04) than EDU (log P = -1.09). In vitro incubation of (5R,6R)-BEEDU with rat whole blood and brain ho‌mogena...

Investigation of the Impact of Foretinib, an Oral Multikinase, on AURKA and AURKB Expression in T98 Glioblastoma Cell Line

Background/Objective: Gliomas are the most common of the primary brain tumors and accounted for more than 40% of all central nervous system (CNS) tumors. Glioblastoma (GBM) remains one of the most fatal human malignancies because of its high angiogenic. Foretinib is an oral multikinase inhibitor that represented antitumor activity in clinical studies.  AURKA and AURKB genes have been shown to b...